Cloning and expression of the neuromedin B receptor and the third subtype of bombesin receptor genes in the mouse

Brain Res. 1997 Jul 11;762(1-2):165-72. doi: 10.1016/s0006-8993(97)00380-6.

Abstract

We cloned the genes for the mouse homologue of the neuromedin B receptor (NMB-R) and the bombesin receptor subtype 3 (BRS-3). Both receptor genes consist of three exons with well-conserved intron-exon borders. Although the NMB-R gene spans more than 10 kb, the BRS-3 gene spans only about 4 kb. Comparison of the mouse and human receptor sequences indicates 90% (NMB-R) and 85% (BRS-3) sequence homology at the amino-acid level. In the adult mouse, the NMB-R mRNA is expressed in the brain, testis, esophagus, intestine and uterus, whereas the BRS-3 mRNA is expressed predominantly in the brain. In the brain, the NMB-R gene expression is prominent in the thalamic and olfactory regions, and the BRS-3 gene is expressed particularly in the hypothalamic region. In mouse testis, the NMB-R gene expression is prominent, and the expression of BRS-3 mRNA is barely detected. In contrast, BRS-3 has been shown to be expressed in rat testis and guinea-pig uterus, therefore it is possible that a different subtype of the bombesin receptor mediates the same response in different species. Together with the mouse GRP-R gene cloned previously, cloning of the mouse NMB-R and BRS-3 genes permits comparison of function and structure of the three bombesin receptor subtypes in the mouse.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Brain Chemistry / physiology
  • Cloning, Molecular
  • Consensus Sequence
  • Gene Expression / physiology
  • Gene Library
  • Male
  • Mice
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • RNA, Messenger / analysis
  • Receptors, Bombesin / analysis
  • Receptors, Bombesin / chemistry
  • Receptors, Bombesin / genetics*
  • Sequence Homology, Amino Acid
  • Testis / chemistry

Substances

  • RNA, Messenger
  • Receptors, Bombesin
  • bombesin receptor subtype 3

Associated data

  • GENBANK/AB010280
  • GENBANK/AB010281