Interleukin-1 beta primes interleukin-8-stimulated chemotaxis and elastase release in human neutrophils via its type I receptor

Eur Cytokine Netw. 1997 Jun;8(2):173-8.


Interleukin-1 (IL-1) is a pleiotropic proinflammatory cytokine which binds to human neutrophils (PMN) and can directly or indirectly activate their functions. In this study we show that a brief exposure to IL-1 beta induces a potentiation of both PMN elastase release and chemotactic response to interleukin-8 (IL-8), the prototype of C-X-C chemokines. Priming by IL-1 beta was maximal at 100 ng/ml, was completely blocked in the presence of IL-1 receptor antagonist (IL-1ra) and, in the chemotaxis assay, was best observed at suboptimal (3-6 ng/ml) or inactive (0.75 ng/ml) concentrations of IL-8. Priming of PMN by IL-1 beta was completely blocked by M1, a specific antibody against the type I IL-1 receptor (IL-1RI). On the other hand M22, an antibody directed against the IL-1 decoy type II IL-1 receptor did not affect IL-1 beta action and slightly increased the priming effect. Thus, exclusively via its type I receptor, IL-1 beta can act on PMN at multiple levels, by promoting their accumulation in tissues through the induction of chemotactic factors (e.g. IL-8) and the upregulation of adhesion molecules, and by priming their response to chemotactic agonists.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Blocking / pharmacology
  • Chemotaxis, Leukocyte / drug effects*
  • Chemotaxis, Leukocyte / physiology
  • Drug Synergism
  • Humans
  • In Vitro Techniques
  • Interleukin-1 / administration & dosage
  • Interleukin-1 / pharmacology*
  • Interleukin-1 / physiology
  • Interleukin-8 / administration & dosage
  • Interleukin-8 / pharmacology*
  • Interleukin-8 / physiology
  • Leukocyte Elastase / metabolism*
  • Neutrophils / drug effects*
  • Neutrophils / enzymology
  • Neutrophils / physiology*
  • Receptors, Interleukin-1 / physiology*
  • Receptors, Interleukin-1 Type I


  • Antibodies, Blocking
  • Interleukin-1
  • Interleukin-8
  • Receptors, Interleukin-1
  • Receptors, Interleukin-1 Type I
  • Leukocyte Elastase