A high level of accumulation of 2-hydroxymuconic 6-semialdehyde from aniline by the transpositional mutant Y-2 of Pseudomonas species AW-2

Microbiol Res. 1997 Jul;152(2):129-35. doi: 10.1016/S0944-5013(97)80003-1.

Abstract

Four transpositional mutants of aniline-assimilating Pseudomonas sp. AW-2 produced 2-hydroxymuconic 6-semialdehyde (HMS) from aniline and accumulated it in a cultural medium. Among the four mutants, strain Y-2 produced the greatest amount of HMS (0.77 mg/ml) from 1 mg/ml of aniline hydrochloride in a 15-h growing culture. The conversion rate of aniline to HMS was 70% on a molar basis. Resting cells of strain Y-2 produced 0.65 mg/ml of HMS during 4h of incubation in a reaction mixture containing 1 mg/ml of aniline hydrochloride (conversion rate, 60%). Transposon Tn5-Mob was found to be inserted into the gene of HMS dehydrogenase in strain Y-2 by sequence analysis.

MeSH terms

  • Alcohol Oxidoreductases / genetics*
  • Aldehyde Oxidoreductases*
  • Amino Acid Sequence
  • Aniline Compounds / metabolism*
  • Base Sequence
  • Cloning, Molecular
  • Fatty Acids, Unsaturated / biosynthesis*
  • Fatty Acids, Unsaturated / chemistry
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Mutation*
  • Pseudomonas / genetics*
  • Pseudomonas / metabolism
  • Sequence Analysis, DNA

Substances

  • Aniline Compounds
  • Fatty Acids, Unsaturated
  • hydroxymuconic semialdehyde
  • Alcohol Oxidoreductases
  • Aldehyde Oxidoreductases
  • 2-hydroxymuconic semialdehyde dehydrogenase
  • aniline