The NAD synthetase NadE (OutB) of Bacillus subtilis is a sigma B-dependent general stress protein

FEMS Microbiol Lett. 1997 Aug 15;153(2):405-9. doi: 10.1111/j.1574-6968.1997.tb12603.x.


The identification of sigma B-dependent general stress proteins is a useful strategy to understand the physiological role of the unspecific stress response in Bacillus subtilis. By N-terminal sequencing of B. subtilis stress proteins Gsp38 was identified as the NAD-synthetase (NadE). NadE was previously characterized as spore outgrowth factor B (OutB) conferring a temperature-sensitive spore outgrowth defective phenotype. Transcriptional studies showed that nadE is strongly induced in response to heat, ethanol and salt stress or after starvation for glucose in a sigma B-dependent manner. Two promoters are involved in transcriptional initiation, the sigma A-dependent upstream promoter contributes to the basal level during growth, whereas the sigma B-dependent downstream promoter is induced after different stress conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacillus subtilis / enzymology
  • Bacillus subtilis / genetics*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / physiology*
  • Base Sequence
  • Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor*
  • Ethanol / pharmacology
  • Gene Expression Regulation, Bacterial / drug effects
  • Gene Expression Regulation, Bacterial / physiology*
  • Glucose / physiology
  • Hot Temperature
  • Ligases / chemistry
  • Ligases / genetics*
  • Molecular Sequence Data
  • Promoter Regions, Genetic / genetics
  • RNA, Bacterial / analysis
  • Sequence Analysis
  • Sigma Factor / genetics
  • Sigma Factor / physiology*
  • Sodium Chloride / pharmacology
  • Transcription, Genetic / genetics


  • Bacterial Proteins
  • RNA, Bacterial
  • SigB protein, Bacteria
  • Sigma Factor
  • Ethanol
  • Sodium Chloride
  • Ligases
  • Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor
  • NAD+ synthase (glutamine-hydrolysing)
  • OutB protein, Bacillus subtilis
  • Glucose