Cloning and Sequencing of a 35.7 Kb in the 70 degree-73 Degree Region of the Bacillus Subtilis Genome Reveal Genes for a New Two-Component System, Three Spore Germination Proteins, an Iron Uptake System and a General Stress Response Protein

Gene. 1997 Jul 31;194(2):191-9. doi: 10.1016/s0378-1119(97)00130-3.


In the framework of the international project aimed at sequencing the Bacillus subtilis (Bs) genome, a 35.7-kb chromosome segment around the pel locus has been cloned and sequenced. This region (35,745 bp; 70 degrees-73 degrees of the genetic map) contains two partial and 38 complete orfs. A homology search for the products deduced from the 39 orfs revealed that 26 of them exhibit significant similarity to known proteins, e.g. germination proteins, sodium-alanine symporter, PTS system, methionine amino peptidase, 2-oxoglutarate/malate translocater, pectate lyase, general stress response protein, RNA helicase, iron uptake and two-component systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / genetics*
  • Bacillus subtilis / physiology
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Bacterial
  • Citrates / metabolism
  • Cloning, Molecular
  • DNA, Bacterial
  • Genes, Bacterial
  • Genome, Bacterial*
  • Iron / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • Open Reading Frames
  • Polysaccharide-Lyases / genetics
  • Spores, Bacterial / genetics


  • Bacterial Proteins
  • Citrates
  • DNA, Bacterial
  • Iron
  • Polysaccharide-Lyases
  • pectate lyase

Associated data

  • GENBANK/D86417