Molecular cloning of a novel receptor-type protein tyrosine phosphatase from murine fetal liver

Gene. 1997 Jul 31;194(2):241-7. doi: 10.1016/s0378-1119(97)00174-1.


A cDNA fragment encoding a novel tyrosine phosphatase (PTPase), termed ptpf, was isolated from day 11.5 mouse fetal liver using reverse transcription-polymerase chain reaction (RT-PCR) with degenerate primers. The 5.5-kb cDNA encoding the complete coding region was isolated from an adult mouse kidney cDNA library. This cDNA contained a single open reading frame (ORF) encoding a predicted 1436-amino-acid protein with a molecular mass of 161,150 Da. Sequence analysis revealed that PTPf was homologous to PTPmu and PTPkappa, and a putative receptor-type PTPase. Northern blotting analysis of adult mouse mRNA indicated the existence of four major ptpf transcripts of approximately 10, 6, 3 and 2.7 kb, and these transcripts were expressed in a tissue-specific manner. During embryogenesis, only the 6-kb transcript was detected.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression
  • Liver / embryology
  • Liver / enzymology*
  • Mice
  • Molecular Sequence Data
  • Protein Tyrosine Phosphatases / biosynthesis
  • Protein Tyrosine Phosphatases / chemistry
  • Protein Tyrosine Phosphatases / genetics*
  • Receptors, Cell Surface / biosynthesis
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • Receptors, Cell Surface
  • Protein Tyrosine Phosphatases

Associated data

  • GENBANK/D88187