Isolation of a mouse cDNA encoding mSTI1, a stress-inducible protein containing the TPR motif

Gene. 1997 Jul 31;194(2):277-82. doi: 10.1016/s0378-1119(97)00206-0.

Abstract

We report the isolation and sequencing of the complete 2079-bp cDNA fragment encoding mSTI1, a murine stress-inducible protein. The predicted ORF encodes a protein of 543 amino acids (aa) and Mr 62,582. The predicted protein has significant homology to stress-inducible proteins from humans (IEF SSP 3521), soybean (GMSTI), yeast (STI1) and a parasite, Leishmania donovani (LSIP). All of these proteins contain 34-aa repeat motifs, termed tetratricopeptide repeats (TPRs), that are proposed to be involved in intra- and intermolecular protein interactions. mSTI1 has ten potential TPR motifs, a putative nuclear localization signal (NLS), six potential phosphorylation sites for casein kinase II and a central proline-rich region. Western analysis detected a protein of approx. 63 kDa in all the major mouse organs and in mouse, monkey and human cell lines.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Western
  • DNA, Complementary
  • Heat-Shock Proteins / chemistry
  • Heat-Shock Proteins / genetics*
  • Humans
  • Mice
  • Molecular Chaperones*
  • Molecular Sequence Data
  • Sequence Analysis, DNA

Substances

  • DNA, Complementary
  • Heat-Shock Proteins
  • Molecular Chaperones
  • STIP1 protein, human
  • Stip1 protein, mouse

Associated data

  • GENBANK/U27830