Clinical significance of tissue inhibitor of metalloproteinase expression in gastric carcinoma

Br J Cancer. 1997;76(4):531-6. doi: 10.1038/bjc.1997.420.


Tissue inhibitor of metalloproteinase (TIMP) has been reported to inhibit tumour invasion through an inactivation of matrix metalloproteinase (MMP) both in vitro and in vivo. Among the TIMP family, TIMP-1 possesses not only proteinase inhibitory activity but also a growth-promoting function. However, the significance of the expression of TIMP-1 in human gastric carcinoma tissue has yet to be clarified. In 50 examined cases of gastric carcinoma, 44 (88%) cases showed a higher expression of TIMP-1 mRNA in the biopsy samples from the tumour tissue (T) than in the biopsy samples from the corresponding normal tissue (N), as determined by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). In a multivariate analysis, the T/N ratio of TIMP-1 mRNA was found to be an independent factor influencing the depth of tumour invasion and was the second most important factor in determining the prognosis of patients. As RT-PCR assay can be performed on biopsy specimens obtained before surgery, an evaluation of the TIMP-1 expression in biopsy specimens by RT-PCR may thus provide useful preoperative information on tumour aggressiveness.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Female
  • Glycoproteins / genetics*
  • Humans
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis*
  • Reproducibility of Results
  • Stomach Neoplasms / metabolism*
  • Tissue Inhibitor of Metalloproteinases


  • Glycoproteins
  • RNA, Messenger
  • Tissue Inhibitor of Metalloproteinases