Objective: To clone retinitis pigmentosa region by yeast artificial chromosome (YAC) and establish the restriction enzyme physical map.
Methods: The ornithine transcarbamoylase (OTC) cDNA probe, which is closely linked to the RP3 locus, was chosen to screen the X chromosome YAC library by colony in situ hybridization. Size determination, sequence taged site (STS) analysis and long range physical mapping were performed with positive YACs. The results obtained were used to map these YACs.
Results: We obtained a 1.6 Mb YAC contig containing information on RP3 range, restriction enzyme sites, CpG islands location and YAC position.
Conclusion: The work provides a good basis for identification and cloning of the RP3 gene.