9-cis-retinoic acid enhances fatty acid-induced expression of the liver fatty acid-binding protein gene

FEBS Lett. 1997 Aug 4;412(3):480-4. doi: 10.1016/s0014-5793(97)00830-2.

Abstract

The role of retinoic acids (RA) on liver fatty acid-binding protein (L-FABP) expression was investigated in the well differentiated FAO rat hepatoma cell line. 9-cis-Retinoic acid (9-cis-RA) specifically enhanced L-FABP mRNA levels in a time- and dose-dependent manner. The higher induction was found 6 h after addition of 10(-6) M 9-cis-RA in the medium. RA also enhanced further both L-FABP mRNA levels and cytosolic L-FABP protein content induced by oleic acid. The retinoid X receptor (RXR) and the peroxisome proliferator-activated receptor (PPAR), which are known to be activated, respectively, by 9-cis-RA and long chain fatty acid (LCFA), co-operated to bind specifically the peroxisome proliferator-responsive element (PPRE) found upstream of the L-FABP gene. Our result suggest that the PPAR-RXR complex is the molecular target by which 9-cis-RA and LCFA regulate the L-FABP gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alitretinoin
  • Animals
  • Carcinoma, Hepatocellular
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / genetics*
  • Dimerization
  • Drug Synergism
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Fatty Acids / pharmacology*
  • Gene Expression Regulation / drug effects*
  • Liver / drug effects
  • Liver / metabolism*
  • Microbodies / metabolism
  • Myelin P2 Protein / biosynthesis
  • Myelin P2 Protein / genetics*
  • Neoplasm Proteins*
  • Nerve Tissue Proteins*
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism
  • Rats
  • Receptors, Cytoplasmic and Nuclear / biosynthesis
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Receptors, Retinoic Acid / metabolism
  • Retinoid X Receptors
  • Transcription Factors / biosynthesis
  • Transcription Factors / metabolism
  • Tretinoin / metabolism
  • Tretinoin / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Carrier Proteins
  • Fabp1 protein, mouse
  • Fabp1 protein, rat
  • Fabp7 protein, rat
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Fatty Acids
  • Myelin P2 Protein
  • Neoplasm Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Retinoic Acid
  • Retinoid X Receptors
  • Transcription Factors
  • Alitretinoin
  • Tretinoin