Expression of PEP carboxylase from Escherichia coli complements the phenotypic effects of pyruvate carboxylase mutations in Saccharomyces cerevisiae

FEBS Lett. 1997 Aug 4;412(3):531-4. doi: 10.1016/s0014-5793(97)00854-5.


We investigated the effects of the expression of the Escherichia coli ppc gene encoding PEP carboxylase in Saccharomyces cerevisiae mutants devoid of pyruvate carboxylase. Functional expression of the ppc gene restored the ability of the yeast mutants to grow in glucose-ammonium medium. Growth yield in this medium was the same in the transformed yeast than in the wild type although the growth rate of the transformed yeast was slower. Growth in pyruvate was slowed down in the transformed strain, likely due to a futile cycle produced by the simultaneous action of PEP carboxykinase and PEP carboxylase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Genetic Complementation Test
  • Genetic Vectors / genetics
  • Mutagenesis*
  • Phenotype
  • Phosphoenolpyruvate Carboxykinase (GTP) / biosynthesis*
  • Phosphoenolpyruvate Carboxykinase (GTP) / genetics*
  • Phosphoenolpyruvate Carboxykinase (GTP) / physiology
  • Pyruvate Carboxylase / genetics*
  • Pyruvate Carboxylase / physiology
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development


  • Phosphoenolpyruvate Carboxykinase (GTP)
  • Pyruvate Carboxylase