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. 1997 Aug 4;412(3):633-6.
doi: 10.1016/s0014-5793(97)00847-8.

K+ Is an Indispensable Cofactor for GrpE Stimulation of ATPase Activity of DnaK X DnaJ Complex From Thermus Thermophilus

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K+ Is an Indispensable Cofactor for GrpE Stimulation of ATPase Activity of DnaK X DnaJ Complex From Thermus Thermophilus

K Motohashi et al. FEBS Lett. .
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Abstract

K+ is an indispensable cofactor for ATPase activity of eukaryotic cytosolic Hsp70 chaperone systems which lack a GrpE homolog. In the case of the bacterial Hsp70 (DnaK) system, GrpE, a nucleotide exchange factor, stimulates ATPase activity but little is known about the effect of K+. Here, we have cloned a grpE gene from a thermophile, Thermus thermophilus, and purified a homodimeric GrpE protein. Using proteins of this bacterium, we found that the GrpE stimulation of ATPase activity of DnaK x DnaJ complex was absolutely dependent on the presence of K+.

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