Antibodies to beta2-glycoprotein I--a specific marker for the antiphospholipid syndrome

Clin Exp Immunol. 1997 Aug;109(2):304-9. doi: 10.1046/j.1365-2249.1997.4601357.x.


The antiphospholipid syndrome is a disorder characterized by recurrent thrombosis and the presence of antibodies specific to phospholipids. However, the diagnosis of this syndrome is hampered by the lack of a specific laboratory test. In this study an ELISA for the measurement of antibodies to solid-phase beta2-glycoprotein I (beta2-GPI) was established and compared with anticardiolipin antibodies for diagnosis of antiphospholipid syndrome. Significantly elevated levels of antibodies to beta2-GPI were found in all patients with definite antiphospholipid syndrome (median = 91 AU). Marginally elevated levels of antibodies to beta2-GPI were observed in 5% of patients with systemic lupus erythematosus (SLE; median = 4 AU), 1% with stroke (median = 3 AU), 13% with infectious mononucleosis (median = 3 AU), 10% with HIV infection (median = 3 AU) and 8% with VDRL false-positive serology for syphilis (median = 4 AU), but not in patients with rheumatoid factor, syphilis or carotid artery stenosis. In contrast, significantly raised levels of anticardiolipin antibodies were observed in 100% of patients with definite antiphospholipid syndrome, 30% with SLE, 88% with HIV infection, 94% with syphilis, 62% with infectious mononucleosis, 9% with rheumatoid factor-positive sera, 74% VDRL false-positive serology for syphilis, 47% with stroke and 0% with carotid artery stenosis. This solid-phase assay for antibodies to beta2-GPI is highly specific for the antiphospholipid syndrome and represents an advance in the laboratory diagnosis of this disorder.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Anticardiolipin / analysis
  • Antiphospholipid Syndrome / diagnosis*
  • Autoantibodies* / analysis
  • Biomarkers / analysis
  • Cerebrovascular Disorders / diagnosis
  • Enzyme-Linked Immunosorbent Assay / methods
  • False Positive Reactions
  • Glycoproteins / immunology*
  • HIV Infections / diagnosis
  • Humans
  • Infectious Mononucleosis / diagnosis
  • Lupus Erythematosus, Systemic / diagnosis
  • Membrane Glycoproteins / immunology*
  • Syphilis / diagnosis
  • beta 2-Glycoprotein I


  • Antibodies, Anticardiolipin
  • Autoantibodies
  • Biomarkers
  • Glycoproteins
  • Membrane Glycoproteins
  • beta 2-Glycoprotein I