Interleukin-6 Secretion in Mice Is Associated With Reduced glucose-6-phosphatase and Liver Glycogen Levels

Am J Physiol. 1997 Aug;273(2 Pt 1):E262-7. doi: 10.1152/ajpendo.1997.273.2.E262.

Abstract

Mice bearing interleukin-6 (IL-6)-secreting tumor were used to study the chronic effect of IL-6 on carbohydrate metabolism. Mice were injected with allogeneic tumor cells transduced with the murine IL-6 gene. Serum IL-6 levels were correlated exponentially with tumor weight. Secretion of IL-6 from the developed tumors was associated with decreased food consumption, reduced body weight, and reduced blood glucose levels. Insulin levels did not change, and 2-deoxyglucose uptake was not affected in most tissues examined. A significant increase of 2-deoxyglucose uptake was measured in the liver. Glycogen content in the liver determined 0, 6, 12, and 18 days after tumor inoculation was 42, 23, 12, and 3 mg/g, respectively. The activity of phosphoenolpyruvate carboxykinase was not affected. The activity of glucose-6-phosphatase (G-6-Phase) determined 6, 12, and 18 days after tumor injection was 84, 70, and 50% of G-6-Pase activity in pair-fed mice bearing nonsecreting tumors, respectively. G-6-Pase mRNA levels were markedly reduced due to inhibition of G-6-Pase gene transcriptional rate.

MeSH terms

  • Animals
  • Blood Glucose / analysis
  • Body Weight / physiology
  • Deoxyglucose / pharmacokinetics
  • Eating / physiology
  • Female
  • Fibrosarcoma / genetics
  • Fibrosarcoma / metabolism
  • Fibrosarcoma / pathology
  • Gene Expression
  • Glucose-6-Phosphatase / genetics
  • Glucose-6-Phosphatase / metabolism*
  • Glycogen / metabolism*
  • Insulin / blood
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism*
  • Interleukin-6 / physiology
  • Liver / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Neoplasm Transplantation
  • Transduction, Genetic

Substances

  • Blood Glucose
  • Insulin
  • Interleukin-6
  • Glycogen
  • Deoxyglucose
  • Glucose-6-Phosphatase