FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes

Nucleic Acids Res. 1997 Sep 15;25(18):3665-71. doi: 10.1093/nar/25.18.3665.


The ability to place a series of gene constructs at a specific site in the genome opens new possibilities for the experimental examination of gene expression and chromosomal position effects. We report that the FLP- FRT site-specific recombination system of the yeast 2mu plasmid can be used to integrate DNA at a chromosomal FRT target site in Drosophila. The technique we used was to first integrate an FRT- flanked gene by standard P element-mediated transformation. FLP was then used to excise the FRT- flanked donor DNA and screen for FLP-mediated re-integration at an FRT target at a different chromosome location. Such events were recovered from up to 5% of the crosses used to screen for mobilization and are easily detectable by altered linkage of a white reporter gene or by the generation of a white + gene upon integration.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA / genetics*
  • DNA Nucleotidyltransferases / genetics*
  • Drosophila / genetics*
  • Gene Expression Regulation
  • Gene Targeting
  • Genes, Insect*
  • Recombination, Genetic*


  • DNA
  • DNA Nucleotidyltransferases
  • FLP recombinase