Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase

Y Kinoshita; K Nishigaki; Y Husimi

doi:10.1093/nar/25.18.3747

Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase

Nucleic Acids Res. 1997 Sep 15;25(18):3747-8. doi: 10.1093/nar/25.18.3747.

Authors

Y Kinoshita¹, K Nishigaki, Y Husimi

Affiliation

¹ Department of Functional Materials Science, Saitama University, 255 Shimo-okubo, Urawa 338, Japan.

Abstract

A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.

MeSH terms

Biotin
DNA / chemistry*
Fluorescent Dyes
Isotope Labeling
Molecular Probe Techniques*
RNA / chemistry*
RNA Ligase (ATP)*

Substances

Fluorescent Dyes
RNA
Biotin
DNA
RNA Ligase (ATP)