Transforming growth factor-beta 1 in asthma. Measurement in bronchoalveolar lavage fluid

Am J Respir Crit Care Med. 1997 Aug;156(2 Pt 1):642-7. doi: 10.1164/ajrccm.156.2.9605065.


Airway wall remodeling is an established pathological feature in asthma. Its causes are not well understood, but one mediator of potential relevance is transforming growth factor-beta 1 (TGF-beta 1). We have measured levels of immunoreactive TGF-beta 1 in bronchoalveolar lavage (BAL) fluid from clinically stable atopic asthmatics and healthy control subjects. We have also examined the influence of allergen exposure on TGF-beta 1 release in the airways using a segmental bronchoprovocation model, with BAL performed at two time points following endobronchial allergen and sham saline challenges. Basal concentrations of TGF-beta 1 were significantly higher in asthmatics than control subjects (median 8.0 versus 5.5 pg/ml, p = 0.027). Following segmental bronchoprovocation, concentrations of TGF-beta 1 at the allergen- and saline-challenged sites were not significantly different after 10 min, (31.3 versus 25.0 pg/ml, p = 0.78), but after 24 h there were significantly higher TGF-beta 1 concentrations at the allergen-challenged sites (46.0 versus 21.5 pg/ml, p = 0.017). We conclude that basal TGF-beta 1 levels in the airways are elevated in atopic asthma and that these levels increase further in response to allergen exposure. These findings are consistent with the hypothesis that TGF-beta 1 is implicated in airway wall remodeling in asthma.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Allergens
  • Asthma / immunology*
  • Asthma / pathology
  • Biopsy
  • Bronchi / pathology
  • Bronchial Provocation Tests / methods
  • Bronchial Provocation Tests / statistics & numerical data
  • Bronchoalveolar Lavage Fluid / chemistry*
  • Bronchoalveolar Lavage Fluid / cytology
  • Bronchoscopy
  • Female
  • Fiber Optic Technology
  • Humans
  • Hypersensitivity, Immediate / immunology
  • Hypersensitivity, Immediate / pathology
  • Male
  • Statistics, Nonparametric
  • Time Factors
  • Transforming Growth Factor beta / analysis*


  • Allergens
  • Transforming Growth Factor beta