Germinal center development

Immunol Rev. 1997 Apr;156:111-26. doi: 10.1111/j.1600-065x.1997.tb00963.x.

Abstract

Using a set of surface markers including IgD and CD38, human tonsillar B cells were classified into discrete subpopulations. Molecular and functional analysis allowed us to identify: i) two sets of naive B cells (Bm1 and Bm2); ii) germinal center founder cells (Bm2'); iii) an obscure population of germinal center B cells, displaying a high load of somatic mutations in IgV genes, C mu to C delta switch and preferential Ig lambda light chain usage: these cells may represent the precursors of normal and malignant IgD-secreting plasma cells; iv) the centroblasts (Bm3) in which somatic mutation machinery is activated; v) the centrocytes (Bm4) in which isotype switch occurs; vi) the memory B cells. The characterization of these subpopulations showed that: i) programmed cell death is set before somatic mutations, possibly providing an efficient way for affinity maturation; ii) only high affinity centrocytes are allowed to switch isotype; iii) CD40-ligation inhibits plasmacytic differentiation of mature B lymphocytes; iv) memory B cells preferentially differentiate into plasma cells; v) IgD isotype switch occurs in normal B cells; vi) receptor editing may be induced by somatic mutations in germinal centers. We also characterized two types of antigen-presenting cells in germinal centers: follicular dendritic cells that select high affinity B cells, and a new subset of germinal center dendritic cells that activate germinal center T cells.

Publication types

  • Review

MeSH terms

  • Animals
  • Apoptosis
  • B-Lymphocyte Subsets / cytology*
  • B-Lymphocyte Subsets / immunology
  • Cell Differentiation
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology
  • Germinal Center / cytology*
  • Germinal Center / immunology
  • Humans
  • Immunophenotyping
  • T-Lymphocytes / immunology