Isolation from fetal bovine serum of a fragment b of complement factor B-like protein improving a long-term survival of human endothelial cells

Arch Biochem Biophys. 1997 Sep 1;345(1):150-5. doi: 10.1006/abbi.1997.0234.

Abstract

It is known that serum is a most important factor supporting cell survival and growth. Particularly, the deprivation of serum would result in the death of human endothelial cell. Our previous paper reported an endothelial cell-viability maintaining factor (EC-VMFa) purified from fetal bovine serum and identified as an apolipoprotein. In the present further study, it is demonstrated that another potent serum factor (refer as EC-VMFb) is also possessed of the endothelial cell-viability maintaining activity, improving a long-term survival of human endothelial cells in serum-free medium. EC-VMFb has a molecular weight of 66,000 (reduced and nonreduced), pI of 4.5 and has been identified as fragment b of complement factor B (Bb)-like protein by amino-terminal amino acid sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Cell Survival / drug effects*
  • Cells, Cultured
  • Complement Factor B / chemistry*
  • Culture Media, Serum-Free
  • Endothelium, Vascular / cytology*
  • Fetal Blood / chemistry*
  • Glycoproteins
  • Humans
  • Isoelectric Point
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Peptide Fragments / chemistry
  • Peptide Fragments / isolation & purification*
  • Peptide Fragments / pharmacology
  • Sequence Homology
  • Umbilical Veins
  • beta 2-Glycoprotein I

Substances

  • Culture Media, Serum-Free
  • Glycoproteins
  • Peptide Fragments
  • beta 2-Glycoprotein I
  • Complement Factor B