The in vitro effect of vitamin D3 analogue EB-1089 on a human prostate cancer cell line (PC-3)

Br J Urol. 1997 Aug;80(2):260-2. doi: 10.1046/j.1464-410x.1997.00261.x.

Abstract

Objective: To determine the effect of vitamin D3 analogue (EB-1089) on the growth and proliferation of a prostate cancer cell line (PC-3).

Materials and methods: PC-3 cells (10(4) cells per well) were plated into 24-well tissue culture plates. After 24 h, the culture medium was replaced with one containing the vitamin D3 analogue EB-1089; a control treatment using only replacement medium was conducted in parallel. Cell proliferation was measured by the incorporation of 3H-thymidine 7 and 12 days after the addition of the vitamin D3 analogue. Cells were precipitated with 5% trichloroacetic acid and the radioactivity determined using a scintillation counter. Each experiment was performed at least five times.

Results: There was a significant dose-dependent inhibition of cell growth after 7 and 12 days of treatment with EB-1089, varying from 40 to 70% of the 3H-thymidine incorporation by controls, respectively. The maximum inhibition occurred with 0.1 micromol/L EB-1089 on day 7 and day 12 (both P < 0.01). Longer incubation times appeared to have a greater effect when higher concentrations of EB-1089 were used.

Conclusion: These in vitro studies have shown that the vitamin D3 analogue EB-1089 can significantly reduce the growth rate of the prostate cancer cell line PC-3. This would support the hypothesis that deficiency of vitamin D increases the risk of prostate cancer and further in vivo testing of vitamin D is warranted for its potential role in active therapy.

MeSH terms

  • Antineoplastic Agents / therapeutic use*
  • Calcitriol / analogs & derivatives*
  • Calcitriol / therapeutic use
  • Cell Division / drug effects
  • Dose-Response Relationship, Drug
  • Humans
  • Male
  • Prostatic Neoplasms / drug therapy
  • Prostatic Neoplasms / pathology*
  • Risk Factors
  • Tumor Cells, Cultured / pathology

Substances

  • Antineoplastic Agents
  • Calcitriol
  • seocalcitol