Expression of serotonin transporter mRNA in rat brain: presence in neuronal and non-neuronal cells and effect of paroxetine

J Chem Neuroanat. 1997 Jul;13(2):71-6. doi: 10.1016/s0891-0618(97)00026-4.

Abstract

The expression of serotonin transporter mRNA in rat brain was-examined by in situ hybridisation. Hybridisation was observed in cells of the known serotonergic nuclei and no other neuronal populations. It was also associated with ependymal cells of the aqueduct which may indicate a specialisation of this part of the ventricular system in anatomical and neurophysiological terms. The effect of the selective serotonin reuptake inhibitor paroxetine on neuronal expression of the serotonin transporter mRNA was examined. Quantitation at the cellular level in the dorsal and median raphe nuclei was carried out by analysis of the mean number of silver grains per cell in autoradiographed sections. No significant change (P > 0.1) in serotonin transporter mRNA expression was observed following 21 day administration of paroxetine (5 mg/kg per day).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Female
  • Gene Expression*
  • In Situ Hybridization
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / metabolism
  • Membrane Transport Proteins*
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Neurons / drug effects
  • Neurons / metabolism*
  • Paroxetine / pharmacology*
  • RNA, Messenger / metabolism*
  • Raphe Nuclei / cytology
  • Raphe Nuclei / drug effects
  • Raphe Nuclei / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Serotonin / metabolism*
  • Serotonin Plasma Membrane Transport Proteins
  • Serotonin Uptake Inhibitors / pharmacology*

Substances

  • Carrier Proteins
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Serotonin Plasma Membrane Transport Proteins
  • Serotonin Uptake Inhibitors
  • Slc6a4 protein, rat
  • Serotonin
  • Paroxetine