Selection of a broad repertoire of CD4+ T cells in H-2Ma0/0 mice

Immunity. 1997 Aug;7(2):187-95. doi: 10.1016/s1074-7613(00)80522-1.


According to past reports, H-2Ma0/0 mice express a single major histocompatiblity complex class II molecule, A(b), heavily loaded with a single peptide derived from the invariant chain, CLIP. Despite the highly restricted diversity of the class II:peptide complexes expressed on thymic stromal cells in the mutant animals, a large and diverse population of CD4+ T cells is positively selected. However, two important issues remained unresolved and are addressed here: Just how preponderant is CLIP occupancy of the class II molecules from H-2M0/0 mice? How extensive and functionally competent is the CD4+ population selected in the mutant animals? Our results argue that a single class II:peptide complex can select a very broad, though not complete, repertoire of CD4+ T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation
  • Antigens, CD / metabolism
  • Antigens, Differentiation, B-Lymphocyte / metabolism
  • Bone Marrow
  • CD4-Positive T-Lymphocytes / classification*
  • CD4-Positive T-Lymphocytes / immunology*
  • Cell Differentiation / immunology
  • Epitopes, T-Lymphocyte / genetics
  • H-2 Antigens / genetics*
  • Histocompatibility Antigens Class II / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Peptides / immunology
  • Radiation Chimera
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Superantigens / metabolism


  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • Epitopes, T-Lymphocyte
  • H-2 Antigens
  • Histocompatibility Antigens Class II
  • Peptides
  • Receptors, Antigen, T-Cell, alpha-beta
  • Superantigens
  • invariant chain