Ocular pulse amplitude (PA) and pulsatile ocular blood flow (POBF) were studied in 80 eyes from 80 subjects with a refractive error between +3.00 and -28.00 dpt using the Langham Ocular Blood Flow System. PA and POBF were correlated with axial length and refractive error using linear regression analysis. A significant correlation (p < 0.001) was found between PA and axial length (r = -0.787), PA and refractive error (r = 0.775), POBF and axial length (r = -0.655) and POBF and refractive error (r = 0.650). Myopic eyes were further divided into subgroups according to axial length, refractive error and fundus oculi characteristics. Each subgroup exhibited a significant reduction in PA (p < 0.001) compared to the control group. POBF reduction was significant in every subgroup except the subgroup with a refractive error lower than 6 dpt and the subgroup with an axial length shorter than 26 mm.