Inhibitors of poly(ADP-ribose) polymerase suppress nuclear fragmentation and apoptotic-body formation during apoptosis in HL-60 cells

FEBS Lett. 1997 Aug 11;413(1):99-103. doi: 10.1016/s0014-5793(97)00887-9.


The effects of 3-aminobenzamide (3ABm) and benzamide (BAm), known specific inhibitors of poly(ADP-ribose) polymerase (PARP), on actinomycin D (Act D)-induced apoptosis in HL-60 cells were examined. These inhibitors had no appreciable effect on apoptotic DNA fragmentation, chromatin condensation or PARP restriction cleavage, but clearly inhibited morphological changes, especially nuclear fragmentation and apoptotic-body formation, in a dose-dependent manner. These results suggest that the synthesis of ADP-ribose polymers is not essential for the progression of apoptotic DNA fragmentation and chromatin condensation, but is required in the processes leading to nuclear fragmentation and the subsequent apoptotic-body formation during apoptosis in HL-60 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminobenzoates / pharmacology
  • Apoptosis / drug effects*
  • Benzamides / pharmacology
  • Benzoates / pharmacology
  • Benzoic Acid
  • Blotting, Western
  • Cell Nucleus / drug effects
  • Cell Nucleus / physiology*
  • DNA Fragmentation / drug effects*
  • Dactinomycin / pharmacology
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / pharmacology
  • HL-60 Cells
  • Humans
  • Microscopy, Electron
  • Microscopy, Fluorescence
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Poly(ADP-ribose) Polymerases / analysis
  • Poly(ADP-ribose) Polymerases / physiology*
  • meta-Aminobenzoates


  • Aminobenzoates
  • Benzamides
  • Benzoates
  • Enzyme Inhibitors
  • Poly(ADP-ribose) Polymerase Inhibitors
  • meta-Aminobenzoates
  • Dactinomycin
  • benzamide
  • 3-aminobenzamide
  • Benzoic Acid
  • Poly(ADP-ribose) Polymerases
  • 3-aminobenzoic acid