Primary structure of matrilin-3, a new member of a family of extracellular matrix proteins related to cartilage matrix protein (matrilin-1) and von Willebrand factor

FEBS Lett. 1997 Aug 11;413(1):129-34. doi: 10.1016/s0014-5793(97)00895-8.


A mouse cDNA encoding for matrilin-3, the third member of the novel matrilin family of extracellular matrix proteins, was cloned. The protein precursor of 481 amino acids consists of a putative signal peptide, a short positively charged sequence, a single vWFA-like domain followed by four epidermal growth factor-like modules and a potential coiled-coil alpha-helical oligomerization domain at the C-terminus. It is the smallest member of the matrilin family with a predicted Mr of the mature protein of 48 902. The primary structure of a C-terminal portion of 310 amino acids of the human matrilin-3 was determined and showed a sequence identity to the mouse matrilin-3 of 84.8%. Northern blot hybridization of mouse matrilin-3 mRNA showed a 2.9 kb mRNA expressed in sternum, femur and trachea and indicates a cartilage-specific expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cartilage Oligomeric Matrix Protein
  • Extracellular Matrix Proteins / chemistry*
  • Extracellular Matrix Proteins / genetics
  • Female
  • Glycoproteins / chemistry*
  • Glycoproteins / genetics
  • Humans
  • Lung / chemistry
  • Matrilin Proteins
  • Mice
  • Mice, Inbred CBA
  • Molecular Sequence Data
  • RNA, Messenger / metabolism
  • Sequence Alignment
  • von Willebrand Factor / chemistry*
  • von Willebrand Factor / genetics


  • Cartilage Oligomeric Matrix Protein
  • Extracellular Matrix Proteins
  • Glycoproteins
  • MATN1 protein, human
  • Matn1 protein, mouse
  • Matn3 protein, mouse
  • Matrilin Proteins
  • RNA, Messenger
  • TSP5 protein, human
  • von Willebrand Factor

Associated data

  • GENBANK/Y10521
  • GENBANK/Y13341