Sequence analysis of Spiroplasma phoeniceum and Spiroplasma kunkelii spiralin genes and comparison with other spiralin genes

Curr Microbiol. 1997 Oct;35(4):240-3. doi: 10.1007/s002849900246.


The spiralin genes from two phytopathogenic spiroplasmas, Spiroplasma phoeniceum and Spiroplasma kunkelii, were amplified by PCR, cloned, and sequenced. Comparison of the amino acid sequences of the five spiralins analyzed to date confirm that the spiralins have a general amphiphilic character and possess a conserved lipoprotein signal peptide. It also shows that a conserved central region and an amino acid repetition, including a VTKXE consensus sequence, are present in all spiralins analyzed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Consensus Sequence
  • Conserved Sequence
  • DNA Primers / genetics
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Protein Sorting Signals / chemistry
  • Protein Sorting Signals / genetics
  • Sequence Homology, Amino Acid
  • Spiroplasma / chemistry
  • Spiroplasma / genetics*


  • DNA Primers
  • Protein Sorting Signals