Cloning and expression of a PR5-like protein from Arabidopsis: inhibition of fungal growth by bacterially expressed protein

Plant Mol Biol. 1997 Aug;34(6):949-59. doi: 10.1023/a:1005893119263.


Pathogenesis-related (PR)-5 proteins are a family of proteins that are induced by different phytopathogens in many plants and share significant sequence similarity with thaumatin. We isolated a complementary DNA (ATLP-3) encoding a PR5-like protein from Arabidopsis which is distinct from two other previously reported PR5 cDNAs from the same plant species. The predicted ATLP-3 protein with its amino-terminal signal sequence is 245 amino acids in length and is acidic with a pl of 4.8. The deduced amino acid sequence of ATLP-3 shows significant sequence similarity with PR5 and thaumatin-like proteins from Arabidopsis and other plants and contains a putative signal sequence at the amino-terminus. The expression of ATLP-3 and a related gene (ATLP-1) that we previously isolated from Arabidopsis was induced by pathogen infection and salicylic acid, a known inducer of pathogenesis-related genes. Southern blot analysis indicates that the ATLP-1 and ATLP-3 are coded by single-copy genes. To study the effect of ATLP-1 and ATLP-3 proteins on fungal growth, the cDNA regions corresponding to putative mature protein were expressed in Escherichia coli and the cDNA encoded proteins were purified. ATLP-1 and ATLP-3 proteins cross-reacted with anti-osmotin and anti-zeamatin antibodies. ATLP-3 protein showed antifungal activity against several fungal pathogens suggesting that ATLP-3 may be involved in plant defense against fungal pathogens.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antifungal Agents / pharmacology*
  • Arabidopsis / genetics*
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Candida albicans / drug effects
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Escherichia coli / genetics
  • Microbial Sensitivity Tests
  • Mitosporic Fungi / drug effects
  • Molecular Sequence Data
  • Plant Diseases / genetics
  • Plant Proteins / biosynthesis
  • Plant Proteins / genetics*
  • Plant Proteins / pharmacology
  • Recombinant Proteins / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sweetening Agents*


  • Antifungal Agents
  • DNA, Complementary
  • Plant Proteins
  • Recombinant Proteins
  • Sweetening Agents
  • pathogenesis-related proteins, plant
  • thaumatin protein, plant

Associated data

  • GENBANK/U83490