Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
, 35 (1-2), 101-13

Physical Mapping of the Rice Genome With YAC Clones

Affiliations
  • PMID: 9291964
Review

Physical Mapping of the Rice Genome With YAC Clones

N Kurata et al. Plant Mol Biol.

Abstract

Construction of a rice physical map covered by YAC clones which have been arranged over half of the genome length is presented here. A total of 1285 RFLP and RAPD markers almost evenly distributed on the rice genetic map could select 2974 YAC clones and 2443 clones of them were located on their original positions. Rice YACs carrying 350 kb average insert fragments of 2443 clones could cover 222 megabase length of the rice genome, corresponding to 52% of the whole genome size (4.3 Mb). Chromosome landing with many YAC clones on the high-density genetic map loci efficiently integrated the genetic map with a physical map. This is the first step to generate a comprehensive genome map of rice. An integrated genome map should be an indispensable tool to figure out genome structure as well as to clone trait genes by map-based cloning.

Similar articles

See all similar articles

Cited by 18 articles

See all "Cited by" articles

References

    1. DNA Res. 1994;1(3):139-48 - PubMed
    1. Methods Enzymol. 1992;216:3-12 - PubMed
    1. Science. 1995 Oct 20;270(5235):480-3 - PubMed
    1. Nat Genet. 1994 Dec;8(4):365-72 - PubMed
    1. Nucleic Acids Res. 1995 Jul 25;23(14):2729-33 - PubMed

LinkOut - more resources

Feedback