Deletion of Glycoprotein gM of Pseudorabies Virus Results in Attenuation for the Natural Host

J Gen Virol. 1997 Sep;78 ( Pt 9):2147-51. doi: 10.1099/0022-1317-78-9-2147.

Abstract

Glycoprotein M (gM) is one of the very few non-essential glycoproteins conserved throughout the herpesvirus family. Despite this conservation little is known about its function in virus replication. To test for the importance of gM in vivo in a natural virus-host system, 6-week-old piglets were intranasally infected with a gM- mutant of the alphaherpesvirus pseudorabies virus (PrV). Following infection virus excretion from the nasal mucosa was decreased ca. 100-fold compared to wild-type or revertant virus. Clinical signs were limited to transiently elevated temperature. In contrast, animals infected by wild-type or revertant virus exhibited high fever, severe respiratory symptoms and affliction of the central nervous system. Prior infection with gM-PrV conferred protection against challenge infection and animals mounted an antibody response against gM after wild-type virus infection. Thus, gM is important for efficient virus replication in vivo and deletion of gM may contribute to development of live attenuated, genetically marked vaccines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Herpesvirus 1, Suid / genetics
  • Herpesvirus 1, Suid / immunology
  • Herpesvirus 1, Suid / pathogenicity*
  • Herpesvirus 1, Suid / physiology
  • Molecular Sequence Data
  • Pseudorabies / prevention & control
  • Pseudorabies / virology*
  • Pseudorabies Vaccines
  • Sequence Deletion
  • Swine
  • Vaccines, Attenuated
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / physiology*
  • Viral Vaccines / genetics
  • Viral Vaccines / therapeutic use
  • Virulence
  • Virus Replication / physiology
  • Virus Shedding

Substances

  • Antibodies, Viral
  • Pseudorabies Vaccines
  • Vaccines, Attenuated
  • Viral Envelope Proteins
  • Viral Vaccines
  • pseudorabies virus glycoproteins

Associated data

  • GENBANK/X97257