In vitro growth-inhibitory activity of pneumocandins L-733,560 and L-743,872 against putatively amphotericin B- and fluconazole-resistant Candida isolates: influence of assay conditions

J Med Vet Mycol. Jul-Aug 1997;35(4):285-7.

Abstract

L-733,560 and L-743,872 are water-soluble pneumocandins with potent antifungal activity. By beginning with the NCCLS M27-T method and varying test format (microdilution vs macrodilution), time of reading (24 h vs 48 h), and test medium (RPMI 1640 vs Antibiotic Medium 3), we have demonstrated that the MICs for these compounds can be altered significantly: the microdilution format, reading after 24 h and use of Antibiotic Medium 3 all reduced the measured MIC. No cross-resistance was demonstrated with either fluconazole or amphotericin B.

MeSH terms

  • Amphotericin B / pharmacology*
  • Anti-Bacterial Agents / pharmacology*
  • Antifungal Agents / pharmacology*
  • Candida / drug effects*
  • Candida / isolation & purification
  • Caspofungin
  • Drug Resistance, Microbial
  • Echinocandins
  • Fluconazole / pharmacology*
  • Humans
  • Lipopeptides
  • Microbial Sensitivity Tests
  • Peptides*
  • Peptides, Cyclic*

Substances

  • Anti-Bacterial Agents
  • Antifungal Agents
  • Echinocandins
  • L 733560
  • Lipopeptides
  • Peptides
  • Peptides, Cyclic
  • Amphotericin B
  • Fluconazole
  • Caspofungin