Rig2, a RING finger protein that interacts with the Kin28/Ccl1 CTD kinase in yeast

Mol Gen Genet. 1997 Aug;255(5):460-6. doi: 10.1007/s004380050518.

Abstract

Kin28/Cell, a cyclin-dependent kinase, is essential for the in vivo phosphorylation of the C-terminal domain of the largest subunit of RNA polymerase II in Saccharomyces cerevisiae. In a search for mutations co-lethal with a thermosensitive kin28 mutation, we have identified genes whose products interact functionally with Kin28. In the present work, we have studied a new complementation group of synthetic lethal mutations. The corresponding gene, RIG2, encodes a predicted RING finger protein. Rig2 is likely to be a homolog of MAT1 of higher eukaryotes which forms a ternary complex with MO15(cdk7) and cyclin H. Our genetic data suggest that Rig2 is a component of transcription factor TFIIH. Transcription activity in a rig2-ts mutant is impeded at restrictive temperature. However, none of the rig2-ts mutants obtained was UV sensitive, suggesting that Rig2 is dispensable for nucleotide excision repair.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Cyclin-Dependent Kinases*
  • DNA Primers / genetics
  • DNA Repair
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Mutation
  • Protein Conformation
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism*
  • Protein-Serine-Threonine Kinases / genetics*
  • Protein-Serine-Threonine Kinases / metabolism*
  • RNA Polymerase II / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / radiation effects
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • TATA-Binding Protein Associated Factors*
  • Transcription Factor TFIID*
  • Transcription Factor TFIIH
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription Factors, TFII*
  • Ultraviolet Rays
  • Zinc Fingers / genetics

Substances

  • Carrier Proteins
  • DNA Primers
  • Saccharomyces cerevisiae Proteins
  • TAF6 protein, S cerevisiae
  • TATA-Binding Protein Associated Factors
  • TFB3 protein, S cerevisiae
  • Transcription Factor TFIID
  • Transcription Factors
  • Transcription Factors, TFII
  • Transcription Factor TFIIH
  • Protein Kinases
  • carboxy-terminal domain kinase
  • Protein-Serine-Threonine Kinases
  • Cyclin-Dependent Kinases
  • Kin28 protein kinase, S cerevisiae
  • RNA Polymerase II