Human dendritic cell responses to lipopolysaccharide and CD40 ligation are differentially regulated by interleukin-10

Eur J Immunol. 1997 Aug;27(8):1848-52. doi: 10.1002/eji.1830270805.


We evaluated the effects of interleukin (IL)-10 on the maturation of human dendritic cells (DC) induced either by lipopolysaccharide (LPS) or CD40 engagement. For this purpose, DC generated by culturing plastic-adherent peripheral blood mononuclear cells for 7 days with granulocyte/macrophage-colony-stimulating factor and IL-4 were incubated for 3 days with either LPS (10 ng/ml) or 3T6 fibroblasts transfected with the gene encoding CD40 ligand, in absence or presence of IL-10. First we found that the membrane expression of CD83, a marker of mature DC, was inhibited by IL-10 when induced by LPS but not by CD40 engagement. Likewise, IL-10 inhibited LPS-induced but not CD40-dependent CD86 (B7.2) up-regulation on DC. Furthermore, IL-10 inhibited the production of IL-8 and tumor necrosis factor-alpha by DC when activated by LPS but not by CD40. In contrast, IL-10 inhibited IL-12 production in both activation systems. We conclude that IL-10 differentially influences LPS-dependent and CD40-dependent pathways of DC maturation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism
  • B7-2 Antigen
  • CD40 Antigens / metabolism*
  • CD40 Ligand
  • Cell Differentiation / drug effects
  • Cell Line
  • Dendritic Cells / cytology
  • Dendritic Cells / drug effects*
  • Dendritic Cells / immunology*
  • Humans
  • Immunoglobulins / biosynthesis
  • In Vitro Techniques
  • Interleukin-10 / pharmacology*
  • Interleukin-12 / biosynthesis
  • Interleukin-8 / biosynthesis
  • Lipopolysaccharides / pharmacology*
  • Membrane Glycoproteins / biosynthesis
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Transfection
  • Tumor Necrosis Factor-alpha / biosynthesis


  • Antigens, CD
  • B7-2 Antigen
  • CD40 Antigens
  • CD83 antigen
  • CD86 protein, human
  • Immunoglobulins
  • Interleukin-8
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • CD40 Ligand
  • Interleukin-12