B cell subpopulations separated by CD27 and crucial collaboration of CD27+ B cells and helper T cells in immunoglobulin production

Eur J Immunol. 1997 Aug;27(8):2073-9. doi: 10.1002/eji.1830270835.


B cell immunoglobulin production is regulated by helper T cells through direct interaction and secreted cytokines. In the present study, we functionally analyzed CD27 in cord and peripheral blood B cells. Adult peripheral blood B cells were separated into CD27+ and CD27- cells, which differed in their morphology. Cord blood B cells did not express CD27, and CD27 expression on peripheral blood B cells increased with age. Only CD27+ B cells had the ability to produce immunoglobulin, which was increased by contact with a tumor necrosis factor-related transmembrane ligand, CD70. Adult peripheral blood CD27+ B cells can be further subdivided into two discrete subtypes: IgD- CD27+ and IgD+ CD27+ B cells. IgD- CD27+ B cells produce IgG, IgM and IgA, whereas IgD+ CD27+ B cells predominantly produce IgM. The addition of activated CD4+ CD45RO T cells expressing CD70 caused down-regulation of CD27 expression on activated B cells, and this down-modulation was completely blocked by anti-CD70 monoclonal antibody, indicating direct T-B cell contact via CD27/CD70. The triggering via CD27 and CD40 additively increased the immunoglobulin production under Staphylococcus aureus Cowan strain plus interleukin-2 stimulation. Taken together, our findings demonstrate that peripheral blood B cells are separated into subpopulations by CD27 and IgD expression and that CD27+ B cells produce large amounts of immunoglobulin by interaction with the CD70 molecule.

MeSH terms

  • Adult
  • Antigens, CD*
  • B-Lymphocyte Subsets / immunology*
  • Base Sequence
  • CD27 Ligand
  • CD4-Positive T-Lymphocytes / immunology
  • CD40 Antigens / metabolism
  • Cell Separation
  • Child
  • Child, Preschool
  • Clone Cells
  • DNA Primers / genetics
  • Fetal Blood / cytology
  • Fetal Blood / immunology
  • Humans
  • Immunoglobulin D / metabolism
  • Immunoglobulins / biosynthesis*
  • In Vitro Techniques
  • Infant
  • Infant, Newborn
  • Leukocyte Common Antigens / metabolism
  • Lymphocyte Cooperation*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Polymerase Chain Reaction
  • T-Lymphocytes, Helper-Inducer / immunology*
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / metabolism*


  • Antigens, CD
  • CD27 Ligand
  • CD40 Antigens
  • CD70 protein, human
  • DNA Primers
  • Immunoglobulin D
  • Immunoglobulins
  • Membrane Proteins
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Leukocyte Common Antigens