The isolation and characterization of a novel G protein-coupled receptor regulated by immunologic challenge

Brain Res. 1997 Aug 1;764(1-2):141-8. doi: 10.1016/s0006-8993(97)00438-1.

Abstract

Using a degenerate PCR based approach, a fragment of the novel G protein-coupled receptor, VTR 15-20, was identified from the rat ventral tegmentum. Hybridization screening and RACE PCR were employed to isolate the full length clone. The cDNA encodes a protein of 305 amino acids which shares homology to several orphan as well as known G protein-coupled receptors. Amino acid analysis demonstrates the VTR 15-20 contains specific regions conserved among the G protein-coupled receptor superfamily. Messenger RNA encoding VTR 15-20 is expressed throughout the mammalian nervous system. Using primary rat culture systems we have demonstrated the expression of VTR 15-20 mRNA in both microglia and astrocytes. The highest levels of VTR 15-20 mRNA expression are detected in peripheral tissues including the spleen. Moreover, we have found that the expression of VTR 15-20 mRNA in brain and spleen is regulated by immunologic challenge. Based on the cellular distribution and regulation by immune challenge and neuronal insult, we hypothesize that VTR 15-20 plays a role in neuroimmune function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Brain Chemistry / drug effects
  • Brain Chemistry / physiology*
  • Cells, Cultured
  • Cloning, Molecular
  • DNA / biosynthesis
  • Excitatory Amino Acid Agonists / pharmacology
  • GTP-Binding Proteins / biosynthesis
  • GTP-Binding Proteins / chemistry*
  • GTP-Binding Proteins / isolation & purification
  • Genomic Library
  • Kainic Acid / pharmacology
  • Lipopolysaccharides / pharmacology
  • Molecular Sequence Data
  • Neuroglia / drug effects
  • Neuroglia / metabolism
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / isolation & purification
  • Rats
  • Receptors, Immunologic / biosynthesis
  • Receptors, Immunologic / chemistry*
  • Receptors, Immunologic / isolation & purification

Substances

  • Excitatory Amino Acid Agonists
  • Lipopolysaccharides
  • RNA, Messenger
  • Receptors, Immunologic
  • DNA
  • GTP-Binding Proteins
  • Kainic Acid

Associated data

  • GENBANK/U76206