Construction of a novel virus that targets HIV-1-infected cells and controls HIV-1 infection

Cell. 1997 Sep 5;90(5):849-57. doi: 10.1016/s0092-8674(00)80350-5.


We describe a recombinant vesicular stomatitis virus lacking its glycoprotein gene and expressing instead the HIV-1 receptor CD4 and a coreceptor, CXCR4. This virus was unable to infect normal cells but did infect, propagate on, and kill cells that were first infected with HIV-1 and therefore had the HIV membrane fusion protein on their surface. Killing of HIV-1-infected cells controlled HIV infection in a T cell line and reduced titers of infectious HIV-1 in the culture by as much as 10(4)-fold. Such a targeted virus could have therapeutic value in reducing HIV viral load. Our results also demonstrate a general strategy of targeting one virus to the envelope protein of another virus to control infection.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CD4 Antigens / genetics
  • Cricetinae
  • GTP-Binding Proteins / genetics
  • Gene Deletion
  • Gene Expression Regulation, Viral / physiology
  • Glycoproteins / genetics
  • HIV Infections / therapy*
  • HIV Infections / virology*
  • HIV-1 / growth & development
  • HIV-1 / physiology*
  • Humans
  • Jurkat Cells / virology
  • Kidney / cytology
  • Membrane Glycoproteins*
  • Membrane Proteins / genetics
  • Microscopy, Immunoelectron
  • Mutagenesis / physiology
  • Receptors, CXCR4
  • Receptors, HIV / genetics
  • Recombinant Fusion Proteins / physiology
  • Vesicular stomatitis Indiana virus / growth & development
  • Vesicular stomatitis Indiana virus / physiology*
  • Vesicular stomatitis Indiana virus / ultrastructure
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / physiology
  • Virus Replication


  • CD4 Antigens
  • G protein, vesicular stomatitis virus
  • Glycoproteins
  • Membrane Glycoproteins
  • Membrane Proteins
  • Receptors, CXCR4
  • Receptors, HIV
  • Recombinant Fusion Proteins
  • Viral Envelope Proteins
  • GTP-Binding Proteins