Gene structure, cDNA sequence, and expression of murine Bak, a proapoptotic Bcl-2 family member

Genomics. 1997 Sep 1;44(2):195-200. doi: 10.1006/geno.1997.4858.


To facilitate the creation of Bak knockout mice and the further analysis of this Bcl-2 family member, we have isolated and sequenced the complete mouse Bak cDNA. The cDNA is 2 kb long and shares an overall nucleotide identity to the human Bak cDNA of 62%. The mouse Bak protein is 208 amino acids long with a predicted molecular weight of 23 kDa. The mouse Bak mRNA could be detected in all mouse tissues examined. In addition we mapped the murine bak gene. It consists of six exons spanning about 10 kb on chromosome 17B. The 5' region of the murine bak gene is unmethylated on the dinucleotide CpG in the area around exon 1. Furthermore, it contains potential binding sites for transcription factors such as Sp1 and c-Myb.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apoptosis / genetics
  • Base Sequence
  • Chromosome Mapping
  • CpG Islands
  • DNA Primers / genetics
  • DNA, Complementary / genetics*
  • Exons
  • Gene Expression
  • Humans
  • In Situ Hybridization, Fluorescence
  • Introns
  • Membrane Proteins / genetics*
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • bcl-2 Homologous Antagonist-Killer Protein


  • BAK1 protein, human
  • Bak1 protein, mouse
  • DNA Primers
  • DNA, Complementary
  • Membrane Proteins
  • bcl-2 Homologous Antagonist-Killer Protein

Associated data

  • GENBANK/Y13231
  • GENBANK/Y13232