Establishment and characterization of cell lines derived from uterine malignant mixed Müllerian tumor

Gynecol Oncol. 1997 Sep;66(3):464-74. doi: 10.1006/gyno.1997.4802.


Objective: We report the establishment and characterization of three new cell lines derived from uterine malignant mixed müllerian tumor (MMMT).

Methods: Three uterine MMMT cell lines from primary tumors of Korean patients were cultured and the involved cell morphology, growth properties, DNA profiles, immunohistochemical properties, tumor-associated antigen secretion, and genetic alterations of related oncogenes and tumor suppressor genes were studied as well.

Results: Three MMMT cell lines were successfully established including one homologous tumor SNU-539 and two heterologous tumors SNU-685 and SNU-1077. All lines showed substrate adherence and high viability and were proven by DNA fingerprinting analysis to be unique. Contamination by mycoplasma and bacteria was excluded. SNU-539 and SNU-1077 cells stained positively for both epithelial and mesenchymal antigens, while SNU-685 cells only stained positively for mesenchymal antigens. The level of secretion of tumor-associated antigens, CA125 and CEA, was shown to be undetectable in all three lines. One missense mutation from AAC to GAC at codon 239 of exon 7 in the p53 gene was identified in SNU-539.

Conclusions: These newly established and characterized permanent uterine MMMT cell lines might be regarded as valuable resources for a multitude of in vitro investigations, which should be used for clarifying the obscure histogenetic origin and understanding the biological behavior of this aggressive tumor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • DNA Fingerprinting
  • Female
  • Genes, p53 / genetics
  • Humans
  • Immunohistochemistry
  • Middle Aged
  • Mixed Tumor, Mullerian / genetics
  • Mixed Tumor, Mullerian / pathology*
  • Mutation
  • Proto-Oncogenes / genetics
  • Tumor Cells, Cultured
  • Uterine Neoplasms / genetics
  • Uterine Neoplasms / pathology*