Genomic organization, sequence, and transcriptional regulation of the human eotaxin gene

Biochem Biophys Res Commun. 1997 Aug 28;237(3):537-42. doi: 10.1006/bbrc.1997.7169.

Abstract

Eotaxin is an eosinophil specific beta-chemokine assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. Its expression is stimulus- and cell-specific. We here describe the genomic organisation (3 exons of 132, 112 and 542 bp and 2 introns of 1211 and 378 bp) and sequence including 3 kb of DNA from the immediate 5' upstream region of the human eotaxin gene. Among the regulatory promoter elements potentially regulating eotaxin gene expression and/or mediating the effects of anti-inflammatory drugs we identified consensus sequences known to interact with nuclear factors like NF-IL6, AP-1, a NF-kappa-B like consensus sequence and gamma-interferon- as well as glucocorticoid response elements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • CCAAT-Enhancer-Binding Proteins
  • Cells, Cultured
  • Chemokine CCL11
  • Chemokines, CC*
  • Chemotactic Factors, Eosinophil / biosynthesis
  • Chemotactic Factors, Eosinophil / chemistry
  • Chemotactic Factors, Eosinophil / genetics
  • Consensus Sequence
  • Cytokines / biosynthesis*
  • Cytokines / chemistry
  • Cytokines / genetics*
  • DNA Primers
  • DNA-Binding Proteins / metabolism
  • Eosinophils / metabolism
  • Exons
  • Gene Expression Regulation*
  • Glucocorticoids / pharmacology
  • Humans
  • Interferon-gamma / pharmacology
  • Introns
  • Keratinocytes / metabolism
  • Molecular Sequence Data
  • NF-kappa B / metabolism
  • Nuclear Proteins / metabolism
  • Organ Specificity
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Rabbits
  • Regulatory Sequences, Nucleic Acid
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Skin / metabolism
  • Transcription Factor AP-1 / metabolism
  • Transcription Factors / metabolism
  • Transcription, Genetic*

Substances

  • CCAAT-Enhancer-Binding Proteins
  • CCL11 protein, human
  • Chemokine CCL11
  • Chemokines, CC
  • Chemotactic Factors, Eosinophil
  • Cytokines
  • DNA Primers
  • DNA-Binding Proteins
  • Glucocorticoids
  • NF-kappa B
  • Nuclear Proteins
  • Transcription Factor AP-1
  • Transcription Factors
  • Interferon-gamma

Associated data

  • GENBANK/Z92709