Structure of IL-13 receptor: analysis of subunit composition in cancer and immune cells

Biochem Biophys Res Commun. 1997 Sep 8;238(1):90-4. doi: 10.1006/bbrc.1997.7248.

Abstract

The structure of IL-13 receptor (IL-13R) is currently under investigation. Recently, two different human IL-13R chains, termed here IL-13R alpha and -alpha' have been cloned. We have examined various cancer and normal cell lines for the presence of mRNA for IL-13R alpha and alpha, as well as IL-4R p140 (termed beta chain) and IL-2R gamma c chains. In renal cell carcinoma, glioblastoma and ovarian carcinoma (IGROV-1) cell lines, both IL-13R alpha and alpha chains were expressed (type I IL-13R). In epidermoid, colon, ovarian adenocarcinoma (PA-1) and normal mouse fibroblast (COS7) cell lines, only IL-13R alpha' was expressed (type II IL-13R). In hematopoietic TF-1 and EBV-immortalized normal B cell lines only IL-13R alpha' but not alpha chain was expressed along with gamma c (type III or type IV IL-13R). IL-13R alpha' chain was faintly detected in human T cells. All cells expressed the IL-4Rp140 beta chain. These data provide a direct support for our model of IL-13R which consists of three different forms composed of different subunits.

MeSH terms

  • Animals
  • B-Lymphocytes / metabolism
  • COS Cells
  • Carcinoma
  • Carcinoma, Squamous Cell
  • Cell Line
  • Cell Line, Transformed
  • Cell Transformation, Viral
  • Colonic Neoplasms
  • Female
  • Glioblastoma
  • Herpesvirus 4, Human
  • Humans
  • Interleukin-13 / metabolism*
  • Interleukin-13 Receptor alpha1 Subunit
  • Kidney Neoplasms
  • Leukemia, Myeloid
  • Ovarian Neoplasms
  • Receptors, Interleukin / biosynthesis
  • Receptors, Interleukin / chemistry*
  • Receptors, Interleukin-13
  • Tumor Cells, Cultured

Substances

  • IL13RA1 protein, human
  • Il13ra1 protein, mouse
  • Interleukin-13
  • Interleukin-13 Receptor alpha1 Subunit
  • Receptors, Interleukin
  • Receptors, Interleukin-13