Gas chromatographic-mass spectrometric identification of main metabolites of stanozolol in cattle after oral and subcutaneous administration

J Chromatogr B Biomed Sci Appl. 1997 Aug 1;695(2):269-77. doi: 10.1016/s0378-4347(97)00124-2.


An analytical method has been developed in order to control the illegal use of stanozolol as growth promoter in livestock. The procedure was based on enzymatic hydrolysis, purification on a Clean Screen DAU column and derivatization with heptafluorobutyric anhydride prior to GC-MS analysis. This method allowed us to study the metabolism of stanozolol in cattle after oral and subcutaneous administrations. Urinary metabolites were identified by mass spectrometry. Stanozolol and 16-hydroxystanozolol were detected after oral administration, while 16-hydroxystanozolol and 4,16-dihydroxystanozolol were found after subcutaneous administration.

Publication types

  • Comparative Study

MeSH terms

  • Administration, Cutaneous
  • Administration, Oral
  • Anabolic Agents / administration & dosage
  • Anabolic Agents / urine*
  • Animals
  • Cattle / metabolism*
  • Gas Chromatography-Mass Spectrometry
  • Stanozolol / administration & dosage
  • Stanozolol / analogs & derivatives
  • Stanozolol / urine*
  • Substance Abuse Detection / veterinary*
  • Veterinary Drugs / administration & dosage
  • Veterinary Drugs / urine*


  • Anabolic Agents
  • Veterinary Drugs
  • 16-hydroxystanozolol
  • 4,16-dihydroxystanozolol
  • Stanozolol