Mass spectrometric quantification of the mu opioid receptor agonist Tyr-D-Arg-Phe-Lys-NH2 (DALDA) in high-performance liquid chromatography-purified ovine plasma

J Chromatogr B Biomed Sci Appl. 1997 Aug 1;695(2):287-98. doi: 10.1016/s0378-4347(97)00182-5.

Abstract

The mu opioid receptor agonist Tyr-D-Arg-Phe-Lys-Amide (D-Arg2-Lys4-Dermorphin(1-4)amide=DALDA) was infused continuously for 2 h into sheep. The presence of DALDA in ovine plasma was determined by reversed-phase high-performance liquid chromatography (RP-HPLC) and mass spectrometry (MS) in plasma samples that were obtained at different times during and following that infusion. A stable isotope-incorporated internal standard, deuterated DALDA (d5-DALDA), was used for the MS quantification of DALDA via the protonated molecule ion, (M+H)+, of DALDA and of d5-DALDA. Time-course data (microg DALDA ml(-1) plasma vs. time) were obtained. Tandem MS (MS-MS) provided the product-ion spectrum of the (M+H)+ ion of DALDA in one of the samples to confirm the amino acid sequence of DALDA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Analgesics / blood*
  • Animals
  • Chromatography, High Pressure Liquid
  • Female
  • Mass Spectrometry
  • Oligopeptides / blood*
  • Oligopeptides / chemistry
  • Pregnancy
  • Receptors, Opioid, mu / agonists*
  • Reproducibility of Results
  • Sheep

Substances

  • Analgesics
  • Oligopeptides
  • Receptors, Opioid, mu
  • tyrosyl-arginyl-phenylalanyl-lysinamide