High-performance liquid chromatographic determination of 4-methylpyrazole in plasma and in dialysate

J Chromatogr B Biomed Sci Appl. 1997 Aug 1;695(2):444-7. doi: 10.1016/s0378-4347(97)00181-3.


A rapid method for the determination of 4-methylpyrazole (4-MP) levels in plasma and in dialysate by isocratic reversed-phase high-performance liquid chromatography with UV detection is described. The internal standard was the 3-methylpyrazole (3-MP). Plasma sample preparation consisted of a protein precipitation. Dialysate samples were injected without preparation. The method was linear up to 30 mg l(-1) in plasma and up to 5 mg l(-1) in dialysate. The within-day precisions (C.V.) were less than 4% in plasma and were less than 2% in dialysate. The day-to-day precisions (C.V.) were less than 7% in plasma and were less than 3% in dialysate. This method is easy to perform and has practical interest for clinicians who need to monitor in emergency 4-MP levels in ethylene glycol and methanol poisonings.

MeSH terms

  • Alcohol Dehydrogenase / antagonists & inhibitors*
  • Chromatography, High Pressure Liquid
  • Dialysis Solutions / analysis*
  • Ethylene Glycol
  • Ethylene Glycols / poisoning
  • Fomepizole
  • Humans
  • Poisoning / drug therapy
  • Pyrazoles / analysis
  • Pyrazoles / blood*
  • Pyrazoles / therapeutic use
  • Renal Dialysis
  • Spectrophotometry, Ultraviolet


  • Dialysis Solutions
  • Ethylene Glycols
  • Pyrazoles
  • Fomepizole
  • Alcohol Dehydrogenase
  • Ethylene Glycol