Enzymatic synthesis of oligosaccharides containing Gal beta-->4Gal disaccharide at the non-reducing end using beta-galactanase from Penicillium citrinum

Biosci Biotechnol Biochem. 1997 Aug;61(8):1258-61. doi: 10.1271/bbb.61.1258.

Abstract

The transglycosylation reaction was done with a beta-galactanase from Penicillium citrinum. The regioselectivity in the transglycosylation reaction was studied using soy bean arabinogalactan as a donor and mono- or disaccharide derivatives containing beta-galactosyl residue as acceptors. We also synthesized oligosaccharides containing Gal beta 1-->4Gal sequence such as Gal beta 1-->4Gal beta1-->4Glc, Gal beta 1-->4Gal beta 1-->3GlcNac, Gal beta 1-->4Gal beta 1-->4GlcNAc, Gal beta 1-->4Gal beta 1-->6GlcNAc, and Gal beta 1-->4Gal beta 1-->3GalNAc for use in the total synthesis of complex sugar chains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Chromatography, High Pressure Liquid
  • Disaccharides / biosynthesis*
  • Glycoside Hydrolases*
  • Glycosylation
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Oligosaccharides / biosynthesis*
  • Penicillium / enzymology*
  • beta-Galactosidase / metabolism*

Substances

  • Disaccharides
  • Oligosaccharides
  • Glycoside Hydrolases
  • beta-galactanase
  • beta-Galactosidase