Molecular cloning and characterization of a cDNA encoding putative phospholipid hydroperoxide glutathione peroxidase from spinach

Biosci Biotechnol Biochem. 1997 Aug;61(8):1379-81. doi: 10.1271/bbb.61.1379.

Abstract

A cDNA encoding spinach putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The cDNA included an open reading frame that encoded a polypeptide of 171 amino acid residues. The deduced amino acid sequence showed about 77 and 50% similarity to plant putative PHGPXs and mammalian PHGPXs, respectively. PCR product with the same size as that of the spinach putative PHGPX were obtained from maize, soybeans, and Arabidopsis, suggesting the expression of putative PHGPX genes in other plants.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / biosynthesis*
  • DNA, Complementary / genetics
  • Glutathione Peroxidase / biosynthesis*
  • Glutathione Peroxidase / genetics
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • Plants / metabolism
  • Polymerase Chain Reaction
  • Soybeans / enzymology
  • Spinacia oleracea / enzymology*
  • Zea mays / enzymology

Substances

  • DNA, Complementary
  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • Glutathione Peroxidase

Associated data

  • GENBANK/D63425