Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells

Science. 1997 Sep 26;277(5334):2005-7. doi: 10.1126/science.277.5334.2005.

Abstract

There is growing evidence that T helper cell subsets (TH1 and TH2) can be differentially recruited to promote different types of inflammatory reactions. Murine TH1 but not TH2 cells are recruited through P- and E-selectin into inflamed tissues, where they induce delayed-type hypersensitivity reactions. The human eotaxin-receptor CCR3, originally described on eosinophils and basophils, was also found to be expressed by TH2 cells. An antibody to CCR3 was used to isolate T cells from peripheral blood that give rise to TH2-polarized cell lines and to identify TH2 cells derived from naïve T cells in vitro. Eotaxin stimulated increases in intracellular calcium and chemotaxis of CCR3(+) T cells. The attraction of TH2 cells by eotaxin could represent a key mechanism in allergic reactions, because it promotes the allergen-driven production of interleukin-4 and interleukin-5 necessary to activate basophils and eosinophils.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Calcium / metabolism
  • Cell Line
  • Cell Separation
  • Chemokine CCL11
  • Chemokines, CC*
  • Chemotaxis, Leukocyte
  • Clone Cells
  • Cytokines / metabolism
  • Cytokines / pharmacology*
  • Humans
  • Interferon-alpha / pharmacology
  • Interferon-gamma / biosynthesis
  • Interleukin-3 / biosynthesis
  • Interleukin-4 / biosynthesis
  • Receptors, CCR3
  • Receptors, Chemokine*
  • Receptors, Cytokine / metabolism*
  • Th2 Cells / metabolism*
  • Th2 Cells / physiology*
  • Transforming Growth Factor beta / pharmacology

Substances

  • CCL11 protein, human
  • CCR3 protein, human
  • Chemokine CCL11
  • Chemokines, CC
  • Cytokines
  • Interferon-alpha
  • Interleukin-3
  • Receptors, CCR3
  • Receptors, Chemokine
  • Receptors, Cytokine
  • Transforming Growth Factor beta
  • Interleukin-4
  • Interferon-gamma
  • Calcium