[Diagnosis of human neurocysticercosis by ELISA-IgG using a purified antigen]

Bol Chil Parasitol. 1996 Jul-Dec;51(3-4):85-90.
[Article in Spanish]


An analysis of immunodiagnosis data for human neurocysticercosis (NC) by ELISA-IgG, complement fixation test (CFT) and indirect hemagglutination test (IHAT) in 67 serum samples and 54 cerebrospinal fluids (CSF) from confirmed cases of different hospitals from the Metropolitan Area of Santiago, Chile, was performed. The cut-off value was determined by using serum samples from 60 apparently healthy persons, whose absorbance values were the mean plus three standard deviations. The sensitivity of ELISA was 97.0% and 100% for serum samples and CSF respectively. This assay was considered of statistical significance (p < 0.05) when it was compared with CFT. Specificity was established by testing a purified antigen over 109 different helminthiasis serum samples, 185 neurological affections other than NC and 60 control samples. A 98.3% of global specificity was found. The use of ELISA-IgG and a purified antigen in the approach of immune diagnosis of NC is considered a useful assay, particularly if it is performed on paired serum/CSF samples.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Animals
  • Antigens, Helminth / immunology*
  • Central Nervous System Diseases / blood
  • Central Nervous System Diseases / cerebrospinal fluid
  • Central Nervous System Diseases / diagnosis*
  • Central Nervous System Diseases / immunology
  • Complement Fixation Tests
  • Cysticercosis / blood
  • Cysticercosis / cerebrospinal fluid
  • Cysticercosis / diagnosis*
  • Cysticercosis / immunology
  • Cysticercus / immunology*
  • Enzyme-Linked Immunosorbent Assay*
  • Humans
  • Immunoglobulin G / immunology*
  • Sensitivity and Specificity


  • Antigens, Helminth
  • Immunoglobulin G