The Staphylococcus Aureus scdA Gene: A Novel Locus That Affects Cell Division and Morphogenesis

Microbiology. 1997 Sep;143 ( Pt 9):2877-82. doi: 10.1099/00221287-143-9-2877.


A new Staphylococcus aureus gene termed scdA was found upstream of the autolysis regulatory genes, lytS and lytR, and was shown to potentially encode a hydrophilic 25 kDa protein. Analysis of scdA transcription revealed that it is transcribed as a monocistronic message and is lytSR-independent. A role in cell wall metabolism was indicated by examination of the scdA mutant S. aureus KB323, which had a grossly aberrant cellular morphology and formed large cell clusters when grown in liquid culture medium. Furthermore, KB323 exhibited a reduced rate of autolysis and had increased peptidoglycan cross-linking compared to the parental strain, NCTC 8325-4. These data suggest that scdA plays an important role in staphylococcal cell division.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Base Sequence
  • Cell Division / genetics
  • Cell Wall / chemistry
  • Cell Wall / genetics
  • DNA, Bacterial / genetics
  • Genes, Bacterial*
  • Microscopy, Electron
  • Molecular Sequence Data
  • Morphogenesis / genetics
  • Mutation
  • Peptidoglycan / chemistry
  • Peptidoglycan / genetics
  • Staphylococcus aureus / cytology
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / growth & development
  • Transcription, Genetic


  • Bacterial Proteins
  • DNA, Bacterial
  • Peptidoglycan

Associated data

  • GENBANK/U57060