Towards the phylogeny of APS reductases and sirohaem sulfite reductases in sulfate-reducing and sulfur-oxidizing prokaryotes

Microbiology (Reading). 1997 Sep;143 ( Pt 9):2891-902. doi: 10.1099/00221287-143-9-2891.

Abstract

The genes for adenosine-5'-phosphosulfate (APS) reductase, aprBA, and sirohaem sulfite reductase, dsrAB, from the sulfur-oxidizing phototrophic bacterium Chromatium vinosum strain D (DSMZ 180(T)) were cloned and sequenced. Statistically significant sequence similarities and similar physicochemical properties suggest that the aprBA and dsrAB gene products from Chr. vinosum are true homologues of their counterparts from the sulfate-reducing chemotrophic archaeon Archaeoglobus fulgidus and the sulfate-reducing chemotrophic bacterium Desulfovibrio vulgaris. Evidence for the proposed duplication of a common ancestor of the dsrAB genes is provided. Phylogenetic analyses revealed a greater evolutionary distance between the enzymes from Chr. vinosum and D. vulgaris than between those from A. fulgidus and D. vulgaris. The data reported in this study are most consistent with the concept of common ancestral protogenotic genes both for dissimilatory sirohaem sulfite reductases and for APS reductases. The aprA gene was demonstrated to be a suitable DNA probe for the identification of apr genes from organisms of different phylogenetic positions. PCR primers and conditions for the amplification of apr homologous regions are described.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Archaea / genetics
  • Archaea / metabolism
  • Base Sequence
  • Chromatium / genetics
  • Chromatium / metabolism
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • Desulfovibrio vulgaris / genetics
  • Desulfovibrio vulgaris / metabolism
  • Evolution, Molecular
  • Genes, Bacterial
  • Hydrogensulfite Reductase
  • Molecular Sequence Data
  • Multigene Family
  • Oxidation-Reduction
  • Oxidoreductases / genetics*
  • Oxidoreductases Acting on Sulfur Group Donors / genetics*
  • Phylogeny*
  • Polymerase Chain Reaction
  • Prokaryotic Cells
  • Sequence Homology, Amino Acid
  • Sulfates / metabolism
  • Sulfur / metabolism

Substances

  • DNA Primers
  • DNA, Bacterial
  • Sulfates
  • Sulfur
  • Oxidoreductases
  • Oxidoreductases Acting on Sulfur Group Donors
  • adenylylsulfate reductase
  • Hydrogensulfite Reductase

Associated data

  • GENBANK/U84759
  • GENBANK/U84760
  • GENBANK/Z69372