Assessment of chromosome 3 copy number in ocular melanoma using fluorescence in situ hybridization

Cancer Genet Cytogenet. 1997 Oct 1;98(1):4-8. doi: 10.1016/s0165-4608(96)00405-0.


Recent reports have indicated that monosomy 3 is a marker of poor prognosis in uveal melanoma. Fluorescence in situ hybridization (FISH) was performed on fresh touch preparations from 17 uveal, and 5 conjunctival melanomas, using the chromosome 3 centromeric probe, D3Z1. Of the 17 uveal melanomas, all of which originated in the choroid, two cases revealed a monosomy of chromosome 3. One of the conjunctival melanomas contained a major clone that was trisomic for chromosome 3, and another conjunctival melanoma contained a tetrasomic population. FISH, using the alpha-satellite probe for chromosome 3 on uveal melanoma imprints, allows one to predict which patients are potentially at a higher risk of relapse. Multiplication, rather than deletion, of copies of chromosome 3 in conjunctival melanomas may be a nonspecific aberration, perhaps indicative of polyploidy, a characteristic of tumor progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Choroid Neoplasms / genetics*
  • Choroid Neoplasms / pathology
  • Chromosomes, Human, Pair 3*
  • Conjunctival Neoplasms / genetics*
  • Conjunctival Neoplasms / pathology
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Melanoma / genetics*
  • Melanoma / pathology
  • Middle Aged