Characterization of a putative retroviral env-related human protein

Pathobiology. 1997;65(3):123-8. doi: 10.1159/000164113.

Abstract

In this study, we developed a rabbit polyclonal antibody to a fusion protein containing the envelope (env) transmembrane (TM) region from the human endogenous retroviral family, HERV-E. We used this reagent to document the expression of TM-related protein by Western blot in endothelial, colon and prostate carcinoma and seminoma cell lines and in peripheral blood mononuclear cells from a healthy donor. We detected a 58-kD protein (as compared to murine TM of 15 kD) that had specificity for the env-related antibodies of the polyclonal antiserum.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / chemistry
  • Animals
  • Antibody Formation
  • Antibody Specificity
  • Colonic Neoplasms / chemistry
  • Endothelium / chemistry
  • Gene Products, env / analysis*
  • Gene Products, env / immunology
  • Humans
  • Male
  • Neoplasm Proteins*
  • Neoplasms / chemistry*
  • Prostatic Neoplasms / chemistry
  • Rabbits
  • Recombinant Fusion Proteins / analysis
  • Recombinant Fusion Proteins / immunology
  • Retroviridae Proteins / analysis*
  • Retroviridae Proteins / immunology
  • Retroviridae*
  • Seminoma / chemistry
  • Testicular Neoplasms / chemistry
  • Tumor Cells, Cultured / chemistry
  • Viral Envelope Proteins / analysis*
  • Viral Envelope Proteins / immunology

Substances

  • Gene Products, env
  • Neoplasm Proteins
  • Recombinant Fusion Proteins
  • Retroviridae Proteins
  • Viral Envelope Proteins
  • retroviral p15E-related protein, human